Vendor-submitted protocol.
Sponsored by
Accuri Cytometers
Published in
November 2009
2009
Flow cytometry is the technique of choice for analysis of mitochondrial transmembrane potential (ΔΨm) in whole cells. JC-1, a cytofluorimetric, lipophilic cationic dye, selectively enters into mitochondria and reversibly changes color from red to green as the membrane potential decreases.
Vendor-submitted protocol.
Published in
November
2009
The Accuri C6 Flow Cytometer System is an ideal platform on which to perform no-wash, differential analysis of human peripheral blood samples. The fixed-voltage PMTs simplify data collection and reduce the potential for data loss due to improper signal amplification.