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DNA/RNA Isolation and Purification
Latest Protocols
The PowerSoil® DNA Isolation Kit is comprised of a novel and proprietary method for isolating genomic DNA from environmental samples utilizing our patented Inhibitor Removal Technology® (IRT). The kit is intended for use with environmental samples containing a high humic acid content including difficult soil types such as compost, sediment, and manure.
From Soil/Environmental Samples
Vendor-submitted protocol. Sponsored by Zymo Research Corporation Published in November 2009
Metagenomics (otherwise known as environmental genomics, ecogenomics, or community genomics) is the study of genetic material recovered directly from environmental samples (e.g., soil). This relatively new field of genetic research focuses on the study of organisms that are not easily cultured in the laboratory and studies of organisms in their natural environment.
From FFPE Archival Tissues
Suzanne Kennedy, Ph.D., Director of R&D Vendor-submitted protocol. Sponsored by Mo Bio Laboratories, Inc. Published in November 2009
Isolation of DNA from paraffin-embedded tissue remains an arduous task for researchers screening hundreds of samples for genetic analysis. Removal of the wax coating encasing the thin layer of tissue and extraction of sufficient intact DNA for use in PCR and qPCR are major obstacles to working with these precious samples.
Environmental Microbiology Extraction
Suzanne Kennedy, Ph.D., Director of R&D Vendor-submitted protocol. Sponsored by Mo Bio Laboratories, Inc. Published in November 2009
Environmental researchers face the difficult task of studying microbial communities from remote locations and under extreme conditions. Two major issues facing soil microbiologists are the preservation of the original microbial population during transport and storage and the removal of enzyme inhibitors from DNA and RNA to ensure successful gene expression or genotyping analysis.
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