DNA/RNA Isolation and Purification
Latest Protocols
From Soil/Environmental Samples
Extraction of inhibitor-free metagenomic DNA from soil
Vendor-submitted protocol. Sponsored by Zymo Research Corporation Published in November 2009
Metagenomics (otherwise known as environmental genomics, ecogenomics, or community genomics) is the study of genetic material recovered directly from environmental samples (e.g., soil). This relatively new field of genetic research focuses on the study of organisms that are not easily cultured in the laboratory and studies of organisms in their natural environment.
From FFPE Archival Tissues
Isolation of DNA from FFPE samples without paraffin removal
Suzanne Kennedy, Ph.D., Director of R&D Vendor-submitted protocol. Sponsored by Mo Bio Laboratories, Inc. Published in November 2009
Isolation of DNA from paraffin-embedded tissue remains an arduous task for researchers screening hundreds of samples for genetic analysis. Removal of the wax coating encasing the thin layer of tissue and extraction of sufficient intact DNA for use in PCR and qPCR are major obstacles to working with these precious samples.
Environmental Microbiology Extraction
Isolation of DNA and RNA from soil using two different methods optimized with Inhibitor Removal Technology® (IRT)
Suzanne Kennedy, Ph.D., Director of R&D Vendor-submitted protocol. Sponsored by Mo Bio Laboratories, Inc. Published in November 2009
Environmental researchers face the difficult task of studying microbial communities from remote locations and under extreme conditions. Two major issues facing soil microbiologists are the preservation of the original microbial population during transport and storage and the removal of enzyme inhibitors from DNA and RNA to ensure successful gene expression or genotyping analysis.


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