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PROTOCOLS
PowerSoil® DNA Isolation Kit

Abstract The PowerSoil® DNA Isolation Kit is comprised of a novel and proprietary method for isolating genomic DNA from environmental samples utilizing our patented Inhibitor Removal Technology® (IRT). The kit is intended for use with environmental samples containing a high humic acid content including difficult soil types such as compost, sediment, and manure. Other more common soil types have also been used successfully with this kit. The isolated DNA has a high level of purity allowing for more successful PCR amplification of organisms from the sample. PCR analysis has been performed to detect a variety of organisms including bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae and Actinomycetes (e.g. Streptomyces).

The PowerSoil® DNA Isolation Kit distinguishes itself from MO BIO’s UltraClean® Soil DNA Isolation Kit with a humic substance/brown color removal procedure. This procedure is effective at removing PCR inhibitors from even the most difficult soil types. Environmental samples are added to a bead beating tube for rapid and thorough homogenization. Cell lysis occurs by mechanical and chemical methods. Total genomic DNA is captured on a silica membrane in a spin column format. DNA is then washed and eluted from the membrane. DNA is then ready for PCR analysis and other downstream applications.

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