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Vendor-submitted protocol. Sponsored by Abcam plc Published in November 2008
The ELISPOT assay is a highly sensitive technique to study cell responses to various drugs, stimuli, and inhibitors and allows the detection of individual cells secreting a particular protein. ELISPOT is often used to investigate the Th1/Th2 response, cancer, vaccine development, viral infection monitoring, infectious diseases, autoimmune diseases and transplantation, among others.
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Cell-free synthesis of functional and endotoxin-free antibody Fab fragments by translocation into microsomes
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