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Electroporation
Featured Protocols
Electroporation
Anjana N. Bhattacharya, Ph.D., and B. Gopalakrishnan, Ph.D. . Vendor-submitted protocol . Sponsored by Mirus Bio LLC . Published in November 2009
Most cell lines of immunological origin are propagated in suspension and are known to be refractory to traditional chemical transfection methods...
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Anjana N. Bhattacharya, Ph.D., and B. Gopalakrishnan, Ph.D. Vendor-submitted protocol. Sponsored by Mirus Bio LLC Published in November 2009
Most cell lines of immunological origin are propagated in suspension and are known to be refractory to traditional chemical transfection methods. Electrotransfection using Ingenio™ Electroporation Solution achieves high-efficiency delivery of plasmid DNA and siRNA into these “hard to transfect” cell lines (Table 1) while maintaining cell viability.
Vendor-submitted protocol. Published in November 2009
Successful electroporation of nucleic acids into primary cells requires determining optimal parameters for each cell type, a process that can be tedious. Factors that affect efficient electroporation of primary cells include waveform, pulse duration, field strength, cells and cell density, nucleic acid concentration and type, and electroporation buffer.
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