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Epigenetics
Latest Protocols
Methylation Detection
Vendor-submitted protocol. Sponsored by Zymo Research Corporation Published in November 2009
High Resolution Melt (HRM) analysis can be used to differentiate DNAs based on variations in primary sequence for applications such as genotyping, SNP analysis, and DNA methylation analysis. Zymo Research has developed the EZ qPCR/HRM PreMix, featuring a robust hot-start polymerase and an intense dsDNA binding dye, for streamlined and precise qPCR and HRM applications.
Antibody-free ChIP
Promega Corporation Vendor-submitted protocol. Sponsored by Promega Corporation Published in May 2009
Figure 1. Schematic diagram of HaloCHIP™ System The HaloChIP™ System is a novel method designed for the covalent capture of intracellular protein:DNA complexes without the use of antibodies that offers an efficient and robust alternative to the standard chromatin immunoprecipitation (ChIP) method.
Methylation Detection
Vendor-submitted protocol. Published in BioTechniques Protocol Guide 2009
Chromatin immunoprecipitation (ChIP) is an established and useful technique for characterizing cellular protein/DNA associations including those specific for genomic loci having undergone histone modification. Consequently, the purity of ChIP DNA is essential for successful downstream analyses (e.g., PCR, Southern blotting) and accurate data interpretation.
DNA Methylation
Vendor-submitted protocol. Published in November 2009
MethylCollector™ provides an efficient method for isolating CpG-methylated DNA from limited amounts of cell or tissue samples. MethylCollector enables rapid screening of the methylation levels across multiple loci, making the method especially useful for analyzing the methylation status of tumor tissues or cells and detecting changes in DNA methylation throughout normal cellular processes.
ChIP
Vendor-submitted protocol. Published in November 2009
Chromatin immunoprecipitation (ChIP) is an important technique for the analysis of chromatin-associated proteins in their native chromatin context. Existing ChIP methods utilize agarose beads to capture the antibody/chromatin complexes, but spin-washing of agarose beads is tedious and slow.
Bisulfate DNA Conversion
Vendor-submitted protocol. Sponsored by Zymo Research Corporation Published in BioTechniques Protocol Guide 2008
The EZ DNA Methylation-Direct™ Kit is a further refinement of our popular EZ DNA Methylation™ and EZ DNA Methylation-Gold™ Kits. The EZ DNA Methylation-Direct™ Kit features simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification.
Epigenetic Modification Analysis
Vendor-submitted protocol. Sponsored by Sequenom Published in BioTechniques Protocol Guide 2008
EpiTYPER is a tool for the discovery and quantitative analysis of DNA methylation that uses base specific cleavage and matrix-assisted later desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Using the speed and accuracy of the MassARRAY® system, this method is ideal for discovery of methylation, for discrimination between methylated and non-methylated samples, and for quantifying the methylation levels of DNA.
Methylation Analysis
Vendor-submitted protocol. Sponsored by Applied Biosystems, part of Life Technologies Published in BioTechniques Protocol Guide 2008
Aberrant DNA methylation patterns have been implicated in many disease systems, including cancer. Bisulfite sequencing is the gold standard in assessing the methylation status of a particular region because only sequencing provides direct detection of methylation events, as well as information across the entire amplified region.
Methylation Analysis
Vendor-submitted protocol. Published in December 2006 2007
DNA methylation patterns—are often considered as a second code, an additional layer of information superimposed on the DNA code that determines many phenotypic attributes.
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