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Methylation Detection
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Methylation Detection
. Vendor-submitted protocol . Sponsored by Zymo Research Corporation . Published in November 2009
High Resolution Melt (HRM) analysis can be used to differentiate DNAs based on variations in primary sequence for applications such as genotyping, SNP analysis, and DNA methylation analysis...
Latest Protocols
Vendor-submitted protocol. Sponsored by Zymo Research Corporation Published in November 2009
High Resolution Melt (HRM) analysis can be used to differentiate DNAs based on variations in primary sequence for applications such as genotyping, SNP analysis, and DNA methylation analysis. Zymo Research has developed the EZ qPCR/HRM PreMix, featuring a robust hot-start polymerase and an intense dsDNA binding dye, for streamlined and precise qPCR and HRM applications.
Vendor-submitted protocol. Published in BioTechniques Protocol Guide 2009
Chromatin immunoprecipitation (ChIP) is an established and useful technique for characterizing cellular protein/DNA associations including those specific for genomic loci having undergone histone modification. Consequently, the purity of ChIP DNA is essential for successful downstream analyses (e.g., PCR, Southern blotting) and accurate data interpretation.
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