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Fermentation
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Fermentation
. Vendor-submitted protocol . Sponsored by New Brunswick Scientific . Published in BioTechniques Protocol Guide 2008
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Vendor-submitted protocol. Sponsored by New Brunswick Scientific Published in BioTechniques Protocol Guide 2008
While use of shake flasks, T-flasks, or roller bottles have become the lab standard in most fermentation and cell culture research labs, lack of controls for pH, dissolved oxygen and other parameters cause an inherent limitation in cell yields and protein expression. When higher yields are needed, rather than inoculate, feed and maintain cultures using dozens or even hundreds of bottles or flasks, a benchtop fermentor or cell-culture bioreactor can be used to increase yields by 10-fold or more.
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