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Gene Expression Analysis
Latest Protocols
Competitive PCR
Vendor-submitted protocol. Sponsored by Sequenom Published in November 2007
MassARRAY® QGE is the ideal complement to fine mapping studies and solution for post-array validation. This methodology is orders of magnitude more sensitive than real-time quantitative PCR and permits very closely related genes to be assayed reliably and quantitatively.
Digital Gene Expression
Vendor-submitted protocol. Published in BioTechniques Protocol Guide 2007 2007
a) Preparation of Digital Expression Libraries Total RNA, converted into double stranded cDNA using standard methods, is cut with a specific restriction enzyme. Sequences adjacent to the cut site are converted into 21 bp tags using a type lls restriction enzyme.
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