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Digital Gene Expression
Latest Protocols
Vendor-submitted protocol. Published in BioTechniques Protocol Guide 2007 2007
a) Preparation of Digital Expression Libraries Total RNA, converted into double stranded cDNA using standard methods, is cut with a specific restriction enzyme. Sequences adjacent to the cut site are converted into 21 bp tags using a type lls restriction enzyme.
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Sub-categories Gene Expression Analysis
Competitive PCR
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