to BioTechniques free email alert service to receive content updates.

Digital Gene Expression
Latest Protocols
Vendor-submitted protocol. Published in BioTechniques Protocol Guide 2007 2007
a) Preparation of Digital Expression Libraries Total RNA, converted into double stranded cDNA using standard methods, is cut with a specific restriction enzyme. Sequences adjacent to the cut site are converted into 21 bp tags using a type lls restriction enzyme.
rightcontent.jsp called otherwise condition rightcontent.jsp called 11111111111111111111
Sub-categories Gene Expression Analysis
Competitive PCR
Search Protocols
Search our protocols for your specific technique.
[]
Submit Your Protocols
BioTechniques is always looking for author-submitted protocols related to peer-reviewed papers, so submit your protocols today to our editorial team. Suppliers or advertisers interested in publishing their protocols should also submit.
Click here for more information
Categories




In order to deliver a personalised, responsive service and to improve the site, we remember and store information about how you use it. This is done using simple text files called cookies which sit on your computer.

By continuing to use this site and access its features, you are consenting to our use of cookies. To find out more about the way Bio Techniques uses cookies please go to our Cookie Policy page.