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Transfection
Latest Protocols
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Some cell lines are considered difficult to transfect because the correct parameters for transfection of that cell line are not known. The reagent:DNA ratio must be tested because laboratories may quantity DNA amounts slightly differently.
Vendor-submitted protocol.
Sponsored by
Invitrogen Corporation, part of Life Technologies
Published in
November
2009
The Neon™ Transfection System is an improved transfection technology designed for highly efficient gene delivery into many cell types that are difficult to transfect, including primary and stem cells. The Neon™ technology uses gold-plated electrodes in a narrow, plastic pipette tip for the electroporation chamber, resulting in a more uniform electric field with minimal pH changes.
Vendor-submitted protocol.
Published in
November
2009
The rat pheochromocytoma PC12 cell line is widely applied as a model system to study a variety of neuronal functions. Upon addition of nerve growth factor (NGF), these cells undergo differentiation characterized by an increase in acetylcholinesterase (AChE) activity, and extension of neurite-like processes.
Vendor-submitted protocol.
Published in
November
2009
Lentiviruses can be used in research applications to transduce a wide range of cell types and stably integrate genetic payload into the host genome, allowing for long-term studies in vivo. This protocol makes use of ® HD Transfection Reagent and newly available components to eliminate toxicity associated with lentiviral preparations and consistently produce titers in the mid to high 1010 transduction units (TU) per milliliter.
Vendor-submitted protocol.
Sponsored by
Roche
Published in
BioTechniques Protocol Guide 2008
Some cell lines are considered difficult to transfect because the correct parameters for transfection of that cell line are not known. The reagent:DNA ratio must be tested because laboratories may quantify DNA amounts slightly differently.
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Sub-categories Gene Transfer
Electroporation
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