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Mutation Scanning/Detection
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Hi-Res Melting curve analysis is a rapid, high-throughput, closed-tube method for mutation scanning and genotyping.PCR-amplified products are subjected to a high-precision denaturation process in the presence of the double-strand DNA binding dye LCGreen® Plus on the Idaho Technology LightScanner® Instrument. Nucleic acid melting is tracked by monitoring the fluorescence of the mixture across a defined temperature range, generating melting profiles that can be used to identify the presence of sequence variation within the amplicon.
Vendor-submitted protocol.
Sponsored by
Idaho Technology
Published in
BioTechniques Protocol Guide 2007
Hi-Res Melting curve analysis is a rapid, high-throughput, closed-tube method for mutation scanning and genotyping. PCR-amplified products are subjected to a high-precision denatuaration process in the presence of the double-strand DNA binding dye LCGreen® Plus on the Idaho Technology LightScanner® Instrument.
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