Vendor-submitted protocol.
Published in
BioTechniques Protocol Guide 2009
Active Motif's Histone Purification Mini Kit enables you to purify the core histone proteins and enrich for histone fractions while preserving their post-translational modifications (e.g., acetylation, methylation, and phosphorylation). This purification method is an improvement over acid precipitation methods and utilizes a convenient spin column and a proprietary buffer system to purify the core histones from cells and tissue samples.