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Histone Purification Mini Kit
Sponsored,vendor-submitted protocol   Published in BioTechniques Protocol Guide 2009 (p.24) DOI: 10.2144/000113016

Active Motif's Histone Purification Mini Kit enables you to purify the core histone proteins and enrich for histone fractions while preserving their post-translational modifications (e.g., acetylation, methylation, and phosphorylation). This purification method is an improvement over acid precipitation methods and utilizes a convenient spin column and a proprietary buffer system to purify the core histones from cells and tissue samples. Histones isolated by this method are suitable substrates for downstream assays and in vitro chromatin assembly.

Unlike standard acid extraction techniques, this kit uses proprietary technology to purify the core histones. Post-translational modifications such as phosphorylation, acetylation, and methylation are preserved, so you can extract core histone proteins from your cell culture or animal model and determine which modifications are present. Purification of histone from yeast cells is also possible with the Histone Purification Mini Kit.

The Histone Purification Mini Kit provides reagents for 20 histone purifications from as little as 8 × 105 cells up to grams of tissue due to the robust histone binding capacity of the purification column. The kit method is simple: first, an extract is made and applied to the purification column and then histones are eluted, enabling the purification of core histones. Histones may be quantified by an OD reading or quantitated empirically on a gel by comparison with histone standards.

Correspondence

Address correspondence to Active Motif North America, 1914 Palomar Oaks Way, Suite 150, Carlsbad, CA 92008, USA. Toll free: (877) 222-9543; Tel.: (760) 431-1263; Fax: (760) 431-1351.

Active Motif Europe, 104 Avenue Franklin Roosevelt, B-1330 Rixensart, Belgium, UK. Free Phone: 0800 169 31 47; France Free Phone: 0800 90 99 79; Germany Free Phone: 0800 181 99 10; Tel.: +32 (0)2 653 0001; Fax: +32 (0)2 653 0050. Active Motif Japan, Azuma Bldg, 7th Floor, 2-21 Ageba-Cho, Shinjuku-KuTokyo, 162-0824, Japan. Tel.: +81 3 5225 3638; Fax: +81 3 5261 8733.

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