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Distinguished photons: the Maestro™ in-vivo fluorescence imaging system
Sponsored,vendor-submitted protocol   Sponsored by Cambridge Research & Instrumentation, Inc. (CRi)    Published in BioTechniques Protocol Guide 2007 (p.49) Abstract

The ability to detect multiple molecular species at once is becoming increasingly important, particularly in vivo.

The ability to detect multiple molecular species at once is becoming increasingly important, particularly in vivo. The advent of novel fluorescent probes has increased the demands on in-vivo fluorescence imaging systems to be able to deftly handle a variety of simultaneous signals. In addition, ubiquitous autofluorescence signals from the skin need to be separated from that of labeled fluorophores to ensure proper quantitative data. To this end, CRi's award-winning Maestro™ multispectral imaging system has been shown to be an effective means of multiplexing and quantitating fluorophores. The sensitivity increase using multispectral imaging is as much as 300 fold, and separating the autofluorescence from the label fluorsecence improves quantitatability and lowers detection limits dramatically. In the NIR, autofluorescence, while still significant, is less of a problem. However, multiplexing multiple fluorophores is still desirable and spectral overlap limits most conventional systems. The multispectral imaging approach used in Maestro allows the separation of as many as five labeled fluorophores from skin autofluorescence, each of which can be quantitated and visualized separately. CRi's partnership with Invitrogen-Molecular Probes® to develop new reagents and software tools for their SAIVI™ (Small Animal In-Vivo Imaging) line of NIR probes makes Maestro an excellent system for both today's problems and tomorrow's. In addition to being simple to use, Maestro can automatically acquire temporal data as rapidly as every few seconds with a spatial resolution from as low as 25 microns to imaging multiple whole mice. Maestro's powerful spectral imaging software tools and quantitation software make it an affordable, easy-to-use system which remains the leader for in-vivo multispectral imaging.


Figure 1. (Click to enlarge)


Sample courtesy Clemens Lowik, Ivo Que, Eric Kaijzel, Andy Waters, Blandine Franke-Fayard and Chris Janse, Leiden University Medical Center.


Figure 2. (Click to enlarge)


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