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PROTOCOLS
5' EndTag™ Nucleic Acid Labeling System from Vector Laboratories (Cat. No. MB-9001)

Vector Laboratories



www.vectorlabs.com

Abstract End labeling is a favored method for applications where an internal label might interfere with hybridization or sequence specific protein binding. The 5' EndTag™ Nucleic Acid Labeling System facilitates the covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags to the 5' end of unmodified oligonucleotides or 5'-OH modified DNA or RNA.

The 5' EndTag™ Kit is ideal for labeling PCR and sequencing primers because a label is attached only at the 5' end, leaving the 3' end available for polymerization. The position of the label does not interfere with hybridization or nucleic acid binding and is, therefore, appropriate for binding of capture probes to affinity matrices and for gel shift assays.

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Other subcategories in the Labeling and Detection
Spectrophotometry/Fluorometry
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