ATPlite™ 1step is a homogenous, luciferase-based luminescence assay system for the quantitative measurement of proliferation and cytotoxicity in cultured mammalian cells. We describe here a protocol for automating the ATPlite 1step system using the JANUS® Cellular Workstation.
cAMP is one of the most important second messengers, mediating diverse physiological responses of neurotransmitters, hormones, and drugs. The intracellular level of cAMP is determined by the balance between the activities of two enzymes: adenylyl cyclase (AC) which synthesizes cAMP from ATP, and phosphodiesterase (PDE) which degrades cAMP to AMP.
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