to BioTechniques free email alert service to receive content updates.

Automated LANCE cAMP Cellular Assays with the JANUS Cellular Workstation
Sponsored,vendor-submitted protocol   Sponsored by Perkinelmer Life Sciences    Published in December 2006 (p.53)

cAMP is one of the most important second messengers, mediating diverse physiological responses of neurotransmitters, hormones, and drugs. The intracellular level of cAMP is determined by the balance between the activities of two enzymes: adenylyl cyclase (AC) which synthesizes cAMP from ATP, and phosphodiesterase (PDE) which degrades cAMP to AMP. The activity of AC is controlled through various G protein-coupled receptors (GPCRs) which are divided into two general classes depending on whether they stimulate or inhibit AC activity. The LANCE cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP levels produced upon modulation of adenylyl cyclase activity by GPCRs. We describe here a protocol for automating cell-based LANCE cAMP assays using cells expressing both classes of GPCRs using the JANUS Cellular Workstation.

The provides a complete, integrated automated system solution ideal for cellular assays in the areas of target identification and validation, assay development, secondary screening, and early ADME/Tox profiling and screening applications.

Figure 1. (Click to enlarge)

PerkinElmer's JANUS Cellular Workstation includes the JANUS Automated Workstation, the EnVision MultiLabel Reader, integrated labware movement and dynamic scheduling with incubation and platewashing options. The system also provides tested, pre-defined assay templates, documented results and application guides. Assay throughput may be quickly and easily scaled up 6 fold or greater as compared to manual methods. Results presented here demonstrate the key performance and pharmacological parameters of the assay with typical Z’ values between 0.80 and 0.90.

More Information
Do you have question about this protocol? Would you like more information about this protocol?
Submit your questions here and Biotechniques will forward your question and contact information from our database to an expert at Perkinelmer Life Sciences who will contact you.
Yes, please forward my question, along with my name, title, company, and e-mail address to Perkinelmer Life Sciences
BioTechniques will forward your question to the company, who will contact you directly by email.
Request more information
Name Question
Job Title
Contact Information
Perkinelmer Life Sciences
549 Albany Street
Boston MA 02118-2512
rightcontent.jsp called otherwise condition rightcontent.jsp called 222222222222222222222
Search Protocols
Search our protocols for your specific technique.
Submit Your Protocols
BioTechniques is always looking for author-submitted protocols related to peer-reviewed papers, so submit your protocols today to our editorial team. Suppliers or advertisers interested in publishing their protocols should also submit.
Click here for more information