to BioTechniques free email alert service to receive content updates.

Membranes & Blotting
Latest Protocols
Western Analysis
Vendor-submitted protocol. Sponsored by Bio-Rad Laboratories Published in November 2008
The most common method for analyzing protein expression levels is Western blotting with detection of a single specific protein target, using horseradish peroxidase– or alkaline phosphatase–conjugated antibody probes combined with colorimetric or chemilumines-cent detection. However, multiplex analyses using these techniques are time-consuming and imprecise.
Western Analysis
Vendor-submitted protocol. Sponsored by Bio-Rad Laboratories Published in November 2007
Western blotting is a widely used and sensitive technique for the detection and characterization of proteins. This technique takes advantage of antibody recognition specificity toward an antigenic sequence in a target protein.
Western Analysis
Vendor-submitted protocol. Sponsored by Pall Life Sciences Published in December 2006
Western blot transfer from an SDS-PAGE gel is a method that can be used to detect individual proteins in a given complex sample. The denatured proteins are resolved by electrophoresis then transferred out of the PAGE gel and onto a microporous membrane, where they are located by stains or by “probing” with specific detection reagents, such as antibodies to the analyte of interest.
rightcontent.jsp called otherwise condition rightcontent.jsp called 11111111111111111111
Sub-categories
Western Analysis
Search Protocols
Search our protocols for your specific technique.
[]
Submit Your Protocols
BioTechniques is always looking for author-submitted protocols related to peer-reviewed papers, so submit your protocols today to our editorial team. Suppliers or advertisers interested in publishing their protocols should also submit.
Click here for more information
Categories