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Microscopy
Latest Protocols
Spinning Disk Microscopy
(Registration Required)
Dynamic live cell experiments such as FRAP (Fluorescence Recovery After Photo-bleaching) require the ability to target concentrated laser illumination onto a user-defi ned ROI (Region Of Interest), while minimizing photo-bleaching associated with sequential image capture. The UltraVIEW┬« PhotoKinesis™ Accessory enables these techniques.
Spinning Disk Microscopy
(Registration Required)
Dynamic live cell experiments such as FRAP (Fluorescence Recovery After Photo-bleaching) require the ability to target concentrated laser illumination onto a user-defined ROI (Region Of Interest), while minimizing photo-bleaching associated with sequential image capture. The UltraVIEW┬« PhotoKinesis™ Accessory enables these techniques.
Multi-Label Techniques for Colocalization Studies
Chris van der Loos, Ph.D. Vendor-submitted protocol. Sponsored by Cambridge Research & Instrumentation, Inc. (CRi) Published in November 2009 2009
Double-staining and even triple-staining with poly- or monoclonal antibodies in immunohistochemistry (IHC) can enhance the study of colocalization—the presence of two or more antigens in one cell. Conventional methods for imaging colocalization, however, are prohibitive because overlapping colors are often indiscernible.
Fluorescence Microscopy
Vendor-submitted protocol. Published in BioTechniques Protocol Guide 2009
With Definite Focus, Carl Zeiss offers a system for the Axio Observer.Z1 that can be used not only for the research of cell cultures in many biomedical applications, for example, cell biology, pharmacology, molecular genetics, developmental biology, and the neurosciences, but also in basic research. The system has been specially designed for fluorescence applications and time-lapse experiments under live cell conditions.
Microscopy and Imaging
Vendor-submitted protocol. Published in BioTechniques Protocol Guide 2008
With Colibri, Carl Zeiss MicroImaging GmbH is introducing an innovative light source that will bring the numerous advantages of LEDs (Light Emitting Diodes) into the fluorescence microscopy domain. LED technology has developed rapidly over recent years in terms not only of available wavelengths but also of emission performance, so that LEDs have come to be used in a wide range of applications.
Confocal Microscopy
Vendor-submitted protocol. Sponsored by Nikon Published in BioTechniques Protocol Guide 2008
Live cell fluorescence imaging is an increasingly popular technique for obtaining dynamic information about cellular processes. However, imaging live cells creates many challenges, not the least of which is keeping the cells alive.
Filters & Filter Sets
Vendor-submitted protocol. Sponsored by Omega Optical Inc. Published in BioTechniques Protocol Guide 2007
Typical organic fluorophores, including Fluorescent Proteins, have excitation and emission spectra that are often overlapping. The peaks of these asymmetric, bell-shaped curves can be quite close together, defining the Stokes shift of that fluorophore.
Microscopy and Imaging
(Registration Required)
Vendor-submitted protocol. Sponsored by Carl Zeiss, Inc. Published in December 2006 2007
How do you increase access to the most demanding Life Sciences applications? The human genome was decoded in 2001, which was a scientific revolution. This prompted a flurry of questions in the scientific community.
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