Simon Walker1, Nicholas Cunniffe1, Martin Bootman1, and H. Llewelyn Roderick1,2
Olympus America Inc.
By employing an advanced imaging system, total internal reflection fluorescence (TIRF) illumination was used to observe the intracellular distribution of STIM-1 labeled structures at the plasma membrane, near-simultaneously with intracellular calcium concentration.
In some respects scientific research has come full circle: Scientists started looking at whole organisms and then drilled down to the molecular level. Now they are beginning to look at the whole organism again, but with a better knowledge of the fundamental molecular building blocks.
As well as the clear technological advances made in recent years, microscopy has also been significantly enhanced by the appearance of new fluorescent proteins. Applications such as FLIP, FRET and FRAP have all greatly improved our understanding of biological pathways.
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