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Live Cell Imaging
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Simon Walker1, Nicholas Cunniffe1, Martin Bootman1, and H. Llewelyn Roderick1,2
Vendor-submitted protocol.
Sponsored by
Olympus America Inc.
Published in
November
2009
By employing an advanced imaging system, total internal reflection fluorescence (TIRF) illumination was used to observe the intracellular distribution of STIM-1 labeled structures at the plasma membrane, near-simultaneously with intracellular calcium concentration.
Vendor-submitted protocol.
Sponsored by
Olympus America Inc.
Published in
BioTechniques Protocol Guide 2008
In some respects scientific research has come full circle: Scientists started looking at whole organisms and then drilled down to the molecular level. Now they are beginning to look at the whole organism again, but with a better knowledge of the fundamental molecular building blocks.
Vendor-submitted protocol.
Sponsored by
Olympus America Inc.
Published in
BioTechniques Protocol Guide 2007
As well as the clear technological advances made in recent years, microscopy has also been significantly enhanced by the appearance of new fluorescent proteins. Applications such as FLIP, FRET and FRAP have all greatly improved our understanding of biological pathways.
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