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PCR
Latest Protocols
HRM (High Resolution Melt)
Vendor-submitted protocol. Sponsored by Bio-Rad Laboratories Published in November 2009
High-resolution melt (HRM) analysis can discriminate nucleotide sequence differences among samples by comparing the differing DNA melting behavior of different sample compositions. HRM offers a cost-effective, yet accurate alternative to probe-based genotyping assays and DNA sequencing (1).
Standard PCR
Vendor-submitted protocol. Published in November 2009
The new heated lid technology, .protect™, featured by the Eppendorf Mastercycler pro, achieves improved evaporation protection even at the corner and edge positions of the thermoblock. The tighter sealing of the newly developed fluid-filled cushion incorporated in the heated lid leads to improved evaporation protection.
Real-time PCR
Vendor-submitted protocol. Sponsored by Bio-Rad Laboratories Published in BioTechniques Protocol Guide 2009
Neuroblastoma is a childhood cancer that is derived from precursor cells of the adrenosympathetic system, originating in the adrenal medulla or sympathetic ganglia. To gain insight into the mechanism of action of the MYCN gene in neuroblastoma pathogenesis, we used RNA interference (RNAi) to suppress MYCN expression.
Real-time PCR
Vendor-submitted protocol. Sponsored by Bio-Rad Laboratories Published in November 2007
Real-time PCR has been used for gene expression analysis for over a decade (Heid et al. 1996, Higuchi et al.
Hot Start PCR
Vendor-submitted protocol. Published in December 2006 2007
A common problem with the polymerase chain reaction is the formation of non-specific products, especially primer-dimers. These unwanted products not only interfere with generating the desired amplicons but also obscure analyses following the reaction.
HRM (High Resolution Melt)
Vendor-submitted protocol. Published in BioTechniques Protocol Guide 2007
DNA melting (dissociation) analysis is widely used for the characterization of DNA amplification products, typically using SYBR® Green I fluorescent dye1,. High Resolution Melt (HRM™) is a recent enhancement to traditional melting analyses that significantly increases the detail and information that can be captured.
Real-time PCR
Vendor-submitted protocol. Sponsored by Applied Biosystems, part of Life Technologies Published in BioTechniques Protocol Guide 2007
Gold standard systems, reagents, and support from Applied Biosystems .
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