Real-time PCR
Latest Protocols
Using Real-time qPCR to Evaluate RNAi-mediated Gene Silencing
Vendor-submitted protocol. Sponsored by Bio-Rad Laboratories Published in BioTechniques Protocol Guide 2009
Neuroblastoma is a childhood cancer that is derived from precursor cells of the adrenosympathetic system, originating in the adrenal medulla or sympathetic ganglia. To gain insight into the mechanism of action of the MYCN gene in neuroblastoma pathogenesis, we used RNA interference (RNAi) to suppress MYCN expression.
Normalization Methods for qPCR
Vendor-submitted protocol. Sponsored by Bio-Rad Laboratories Published in November 2007
Real-time PCR has been used for gene expression analysis for over a decade (Heid et al. 1996, Higuchi et al.
Complete Real-Time PCR Solutions
Vendor-submitted protocol. Sponsored by Applied Biosystems, part of Life Technologies Published in BioTechniques Protocol Guide 2007
Gold standard systems, reagents, and support from Applied Biosystems .


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