Published in
BioTechniques Protocol Guide 2007
(p.71)
Abstract
Gold standard systems, reagents, and support from Applied Biosystems
Gold standard systems, reagents, and support from Applied Biosystems
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* RNA Isolation kits available through Ambion, an Applied Biosystems company
The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from total RNA. While designed for short or long term quality cDNA archiving, the kit generates high cDNA yields from 0.02 to 2 µg of total RNA. Downstream applications include real-time PCR, standard PCR and microarray analysis. 
TaqMan® Universal PCR Master Mix**
** Contains AmpliTaq® Gold DNA Polymerase, the world's most referenced brand of DNA polymerase contains all components needed – enzyme, buffer, dNTPs – for all 5′ nuclease assays including TaqMan® Gene Expression Assays. This easy-to-use solution uses universal cycling conditions enabling you to run multiple assays on a plate and employs AmpliTaq Gold® DNA polymerase for enhanced performance.
TaqMan® Low Density Arrays are customizable 384-well micro fluidic cards that perform hundreds of real-time PCR reactions simultaneously. Simple to implement, TaqMan® Arrays require minimal sample amounts and run 1–8 samples in parallel against 12–384 preloaded TaqMan® Gene Expression Assays without liquid-handling robotics or complex pipetting.
The Applied Biosystems 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 96-and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading—and now also offers optional Fast real-time PCR capability.
The advanced SDS v2.3 software for the Applied Biosystems 7900HT Fast Real-Time PCR System has many features such as RQ Manager that simultaneously compares expression levels of 10 plates and makes it easy to visualize results. The software also includes flags and filters for increased quality control, problem notification, filtering and troubleshooting.
* RNA Isolation kits available through Ambion, an Applied Biosystems company
** Contains AmpliTaq® Gold DNA Polymerase, the world's most referenced brand of DNA polymerase
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