to BioTechniques free email alert service to receive content updates.

protocol
Complete Real-Time PCR Solutions
Sponsored,vendor-submitted protocol   Sponsored by Applied Biosystems, part of Life Technologies    Published in BioTechniques Protocol Guide 2007 (p.71) Abstract

Gold standard systems, reagents, and support from Applied Biosystems

Gold standard systems, reagents, and support from Applied Biosystems





*

* RNA Isolation kits available through Ambion, an Applied Biosystems company

The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from total RNA. While designed for short or long term quality cDNA archiving, the kit generates high cDNA yields from 0.02 to 2 µg of total RNA. Downstream applications include real-time PCR, standard PCR and microarray analysis.





TaqMan® Universal PCR Master Mix**

** Contains AmpliTaq® Gold DNA Polymerase, the world's most referenced brand of DNA polymerase contains all components needed – enzyme, buffer, dNTPs – for all 5′ nuclease assays including TaqMan® Gene Expression Assays. This easy-to-use solution uses universal cycling conditions enabling you to run multiple assays on a plate and employs AmpliTaq Gold® DNA polymerase for enhanced performance.





TaqMan® Low Density Arrays are customizable 384-well micro fluidic cards that perform hundreds of real-time PCR reactions simultaneously. Simple to implement, TaqMan® Arrays require minimal sample amounts and run 1–8 samples in parallel against 12–384 preloaded TaqMan® Gene Expression Assays without liquid-handling robotics or complex pipetting.





The Applied Biosystems 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 96-and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading—and now also offers optional Fast real-time PCR capability.





The advanced SDS v2.3 software for the Applied Biosystems 7900HT Fast Real-Time PCR System has many features such as RQ Manager that simultaneously compares expression levels of 10 plates and makes it easy to visualize results. The software also includes flags and filters for increased quality control, problem notification, filtering and troubleshooting.

* RNA Isolation kits available through Ambion, an Applied Biosystems company

** Contains AmpliTaq® Gold DNA Polymerase, the world's most referenced brand of DNA polymerase

More Information
 
Do you have question about this protocol? Would you like more information about this protocol?
Submit your questions here and Biotechniques will forward your question and contact information from our database to an expert at Applied Biosystems, part of Life Technologies who will contact you.
Yes, please forward my question, along with my name, title, company, and e-mail address to Applied Biosystems, part of Life Technologies
BioTechniques will forward your question to the company, who will contact you directly by email.
Request more information
 
Name Question
Job Title
Company
Email
Contact Information
Applied Biosystems, part of Life Technologies
850 Lincoln Centre Drive
Foster City CA 94404
USA
Tel:(800) 345-5224
Protocol Categories
rightcontent.jsp called otherwise condition rightcontent.jsp called 222222222222222222222
Search Protocols
Search our protocols for your specific technique.
Submit Your Protocols
BioTechniques is always looking for author-submitted protocols related to peer-reviewed papers, so submit your protocols today to our editorial team. Suppliers or advertisers interested in publishing their protocols should also submit.
Click here for more information