to BioTechniques free email alert service to receive content updates.

protocol
NucleoBond® Xtra Midi/Maxi, the new generation of anion exchangers
Sponsored,vendor-submitted protocol   Sponsored by Macherey Nagel GmbH& Co .KG    Published in BioTechniques Protocol Guide 2007 (p.73)

Kits

  • NucleoBond® Xtra Midi, typical yield ≥ 250 µg plasmid DNA

  • NucleoBond® Xtra Maxi, typical yield ≥ 1000 µg plasmid DNA

  • NucleoBond® Xtra Midi/Maxi Plus, NucleoBond® Finalizer/Finalizer Large included to speed up DNA precipitation. Saving of time!




Fig. 1.


Features

  • the new generation of anion exchangers

  • up to 60% time saving

  • up to 100% increased yields of transfection-grade plasmid DNA

  • highest purity of plasmid DNA due to anion-exchange technology

MACHEREY-NAGEL has developed NucleoBond® Xtra, a new generation of anion exchangers based on the patented NucleoBond® technology.

With NucleoBond® Xtra typical yields of ≥ 250 µg (Midi) or ≥1000 µg (Maxi) of ultra-pure plasmid DNA can be obtained in about half of the time compared to other Midi and Maxi kits based on anion-exchange chromatography.


Fig. 1. (Click to enlarge)


Kit

Preps

Cat. No.

NucleoBond® Xtra Midi

10/50/100

740410.10/.50/.100

NucleoBond® Xtra Mixi Plus

10/50

740412.10/.50

NucleoBond® Xtra Midi

10/50/100

740414.10/.50/.100

NucleoBond® Xtra Maxi Plus

10/50

740416.10/.50

NucleoBond® Xtra Midi and Maxi kits contain enlarged columns which leads to lower silica resin beds. This in turn enables a faster flow of lysate and buffers through the columns.

Specially designed column filters are included for convenient and time-saving clarification of bacterial lysates. The column filters are supplied directly inserted in the NucleoBond® Xtra columns and allow parallel clarification of bacterial lysate and loading onto the column. Their large, structured surface leads to high filter flow rates and minimized risk of clogging.

The improved silica material with greater DNA binding capacity is based on the proven NucleoBond® anion-exchanger group MAE (methylaminoethanol). Optimized buffer compositions additionally lead to improved alkaline lysis and increased column flow rates.

Procedure

Bacteria are harvested from an overnight culture and lysed by an optimized alkaline lysis procedure. The bacterial lysate is cleared and loaded onto the equilibrated column in one step! The 1st washing step is performed with inserted column filter in order to obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation (NucleoBond® Xtra Midi or Maxi) or speeded-up by the use of the NucleoBond® Finalizer/Finalizer Large included in the NucleoBond® Xtra Midi Plus/Maxi Plus kits.


Fig. 2. (Click to enlarge)


More Information
 
Do you have question about this protocol? Would you like more information about this protocol?
Submit your questions here and Biotechniques will forward your question and contact information from our database to an expert at Macherey Nagel GmbH& Co .KG who will contact you.
Yes, please forward my question, along with my name, title, company, and e-mail address to Macherey Nagel GmbH& Co .KG
BioTechniques will forward your question to the company, who will contact you directly by email.
Request more information
 
Name Question
Job Title
Company
Email
Contact Information
Macherey Nagel GmbH& Co .KG
Protocol Categories
rightcontent.jsp called otherwise condition rightcontent.jsp called 222222222222222222222
Search Protocols
Search our protocols for your specific technique.
Submit Your Protocols
BioTechniques is always looking for author-submitted protocols related to peer-reviewed papers, so submit your protocols today to our editorial team. Suppliers or advertisers interested in publishing their protocols should also submit.
Click here for more information