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Protein Extraction Isolation and Purification
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Protein Extraction Isolation and Purification
. Vendor-submitted protocol . Sponsored by Promega Corporation .
The HaloTag® Protein Purification System is based on a unique protein tag, HaloTag®, which allows covalent, efficient, and specific capture of proteins expressed in Escherichia coli...
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Vendor-submitted protocol. Sponsored by Promega Corporation
The HaloTag® Protein Purification System is based on a unique protein tag, HaloTag®, which allows covalent, efficient, and specific capture of proteins expressed in Escherichia coli. HaloTag® was engineered from a bacterial dehalogenase to enhance expression and solubility of recombinant proteins and to provide efficient protein purification coupled with tag removal.
Vendor-submitted protocol. Published in November 2009
The tough exterior cuticle of Caenorhabditis elegans makes the nematode resilient to lysis and impedes proteomic analyses, particularly when preservation of the native conformation and biological activity of proteins prohibits the use of chaotropes or detergents. Total disruption of nematodes resulting in maximal protein yields from mild, non-denaturing buffers were obtained using the PCT Shredder device followed by pressure cycling technology (PCT).
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