The HaloTag® Protein Purification System is based on a unique protein tag, HaloTag®, which allows covalent, efficient, and specific capture of proteins expressed in Escherichia coli. HaloTag® was engineered from a bacterial dehalogenase to enhance expression and solubility of recombinant proteins and to provide efficient protein purification coupled with tag removal.
The tough exterior cuticle of Caenorhabditis elegans makes the nematode resilient to lysis and impedes proteomic analyses, particularly when preservation of the native conformation and biological activity of proteins prohibits the use of chaotropes or detergents. Total disruption of nematodes resulting in maximal protein yields from mild, non-denaturing buffers were obtained using the PCT Shredder device followed by pressure cycling technology (PCT).
rightcontent.jsp called otherwise condition
Search our protocols for your specific technique.
Submit Your Protocols
BioTechniques is always looking for author-submitted protocols related to peer-reviewed papers, so submit your protocols today to our editorial team. Suppliers or advertisers interested in publishing their protocols should also submit.
Click here for more information
In order to deliver a personalised, responsive service and to improve the site, we remember and store information about how you use it. This is done using simple text files called cookies which sit on your computer.