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RNAi_Protocols
Latest Protocols
Small RNA
Vendor-submitted protocol. Sponsored by Applied Biosystems, part of Life Technologies Published in BioTechniques 2010 Protocol Guide
Lipofectamine┬« RNAiMAX Transfection Reagent is a proprietary, animal origin–free formulation for the transfection of siRNA into eukaryotic cells with low cytotoxicity. Silencer┬« Select siRNAs are high-performing siRNA molecules that incorporate the latest innovations in siRNA design, chemical modifications, and off-target effect prediction algorithms.
siRNA
Vendor-submitted protocol. Sponsored by Invitrogen Corporation, part of Life Technologies Published in BioTechniques 2010 Protocol Guide
43 - pp
Stealth RNAi™ siRNAs are highly potent, specific, and stable siRNA duplexes that are particularly suited for in vivo applications. This protocol describes the in vivo delivery of Stealth RNAi™ siRNA to mouse brain using an osmotic minipump.
siRNA
Vendor-submitted protocol. Sponsored by Invitrogen Corporation, part of Life Technologies Published in November 2009
Lipofectamine™ RNAiMAX Reagent is a proprietary formulation specifically developed for highly efficient delivery of Stealth™ RNAi or short interfering RNA (siRNA) to mammalian cells for RNAi analysis. This synopsis provides a recommended procedure to transfect Stealth™ RNAi or siRNA into human umbilical vein endothelial cells (HUVECs) using Lipofectamine™ RNAiMAX.
Small RNA
Vendor-submitted protocol. Sponsored by Applied Biosystems, part of Life Technologies Published in November 2009
The SOLiD™ System provides researchers with a highly sensitive method for genome-wide analysis of RNAs without the probe bias of microarrays. Using the SOLiD™ Small RNA Expression Kit (SREK), researchers can now generate small RNA libraries in a single day with a simple, easy-to-use protocol that preserves the strand information of the original molecule.
microRNA
Vendor-submitted protocol. Sponsored by Exiqon A/S Published in November 2008
This protocol is for a microRNA profile using the miRCURY™ LNA Arrays with the Tecan HS4800™ Pro Hybridization Station. Protocols for manual hybridization are available at Web site (external).
siRNA
Vendor-submitted protocol. Published in November 2008
The full length version of this protocol, authored by Daniel R. Clayburgh and Jerrold R.
siRNA
Vendor-submitted protocol. Sponsored by Integrated DNA Technologies BTN Published in December 2006 2007
SiRNA is widely used for analyzing gene function and target validation in vitro and in recent years has been successfully extended to in vivo research. Integrated DNA Technologies (IDT) synthesizes siRNAs for both in vitro and in vivo applications.
microRNA
Vendor-submitted protocol. Sponsored by Exiqon A/S Published in BioTechniques Protocol Guide 2007
Acknowledgement for the development of these protocols: Dr. Erno Wienholds and Dr.
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