Stealth RNAi™ siRNAs are highly potent, specific, and stable siRNA duplexes that are particularly suited for in vivo applications. This protocol describes the in vivo delivery of Stealth RNAi™ siRNA to mouse brain using an osmotic minipump.
Lipofectamine™ RNAiMAX Reagent is a proprietary formulation specifically developed for highly efficient delivery of Stealth™ RNAi or short interfering RNA (siRNA) to mammalian cells for RNAi analysis. This synopsis provides a recommended procedure to transfect Stealth™ RNAi or siRNA into human umbilical vein endothelial cells (HUVECs) using Lipofectamine™ RNAiMAX.
SiRNA is widely used for analyzing gene function and target validation in vitro and in recent years has been successfully extended to in vivo research. Integrated DNA Technologies (IDT) synthesizes siRNAs for both in vitro and in vivo applications.
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