siRNA
Latest Protocols
Stealth RNAi™ siRNA brain infusion
Vendor-submitted protocol. Sponsored by Invitrogen Corporation, part of Life Technologies Published in BioTechniques 2010 Protocol Guide
43 - pp
Stealth RNAi™ siRNAs are highly potent, specific, and stable siRNA duplexes that are particularly suited for in vivo applications. This protocol describes the in vivo delivery of Stealth RNAi™ siRNA to mouse brain using an osmotic minipump.
Transfecting Stealth™ RNAi or siRNA into HUVEC Cells Using Lipofectamine™ RNAiMAX
Vendor-submitted protocol. Sponsored by Invitrogen Corporation, part of Life Technologies Published in November 2009
Lipofectamine™ RNAiMAX Reagent is a proprietary formulation specifically developed for highly efficient delivery of Stealth™ RNAi or short interfering RNA (siRNA) to mammalian cells for RNAi analysis. This synopsis provides a recommended procedure to transfect Stealth™ RNAi or siRNA into human umbilical vein endothelial cells (HUVECs) using Lipofectamine™ RNAiMAX.
Transfection of Caco-2 Cells with Using the siLentFect™ Lipid Reagent
Vendor-submitted protocol. Published in November 2008
The full length version of this protocol, authored by Daniel R. Clayburgh and Jerrold R.
Producing siRNA for in vivo applications
Vendor-submitted protocol. Sponsored by Integrated DNA Technologies BTN Published in December 2006 2007
SiRNA is widely used for analyzing gene function and target validation in vitro and in recent years has been successfully extended to in vivo research. Integrated DNA Technologies (IDT) synthesizes siRNAs for both in vitro and in vivo applications.


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