Christopher Grefen, and Michael R. Blatt
We have devised a novel, Gateway-compatible cloning system named 2in1 that enables single-step transfer of two independent genes into two different expression cassettes on the same vector backbone.
Markéta Častorálová, Tomáš Ruml, and Zdeněk Knejzlík
Small ubiquitin-related modifier-2/3 (SUMO-2/3) is a member of the ubiquitin-like (Ubl) protein family. Conjugation of SUMO-2/3 to target proteins is influenced by various stress conditions and chemical inhibitors.
Shereen Basiouni, Herbert Fuhrmann, and Julia Schumann
Transfection of suspension cells has proven to be very difficult using conventional methods.
Jun Arii ,
and Wesley I. Sundquist
Transient transfection of small interfering RNA (siRNA) provides a powerful approach for studying cellular protein functions, particularly when the target protein can be re-expressed from an exogenous siRNA-resistant construct in order to rescue the kno.
Red/ET recombination with chimeric oligonucleotides allows rapid generation of BAC transgenes harboring full-length or truncated huntingtin cDNA
Stefanie Hager, Saskia Lösch, Stephan Noll, Loren Khan-Vaughan, Michelle E. Ehrlich, and Harald Kranz
Huntington's disease (HD) is a fatal neurodegenerative disorder that is caused by a CAG repeat expansion encoding a polyglutamine tract in the huntingtin (htt) gene.