Christopher Grefen, and Michael R. Blatt
We have devised a novel, Gateway-compatible cloning system named 2in1 that enables single-step transfer of two independent genes into two different expression cassettes on the same vector backbone.
Markéta Častorálová, Tomáš Ruml, and Zdeněk Knejzlík
Small ubiquitin-related modifier-2/3 (SUMO-2/3) is a member of the ubiquitin-like (Ubl) protein family. Conjugation of SUMO-2/3 to target proteins is influenced by various stress conditions and chemical inhibitors.
Shereen Basiouni, Herbert Fuhrmann, and Julia Schumann
Transfection of suspension cells has proven to be very difficult using conventional methods.
Jun Arii ,
and Wesley I. Sundquist
Transient transfection of small interfering RNA (siRNA) provides a powerful approach for studying cellular protein functions, particularly when the target protein can be re-expressed from an exogenous siRNA-resistant construct in order to rescue the kno.
Red/ET recombination with chimeric oligonucleotides allows rapid generation of BAC transgenes harboring full-length or truncated huntingtin cDNA
Stefanie Hager, Saskia Lösch, Stephan Noll, Loren Khan-Vaughan, Michelle E. Ehrlich, and Harald Kranz
Huntington's disease (HD) is a fatal neurodegenerative disorder that is caused by a CAG repeat expansion encoding a polyglutamine tract in the huntingtin (htt) gene.
PCR-based screening of targeted mutants for the fast and simultaneous identification of bacterial virulence factors
Ana Henriques, Filipe Carvalho
Rita Pombinho #,
and Didier Cabanes
Understanding the strategies used by pathogens to infect, survive, and proliferate in their hosts requires the identification of virulence factors.
Transformation of diatom Phaeodactylum tricornutum by electroporation and establishment of inducible selection marker
Zhi-Kai Yang ,
and Hong-Ye Li
Diatoms are important primary producers in the marine ecosystem. Currently it is difficult to genetically transform diatoms due to the technical limitations of existing methods.
Olga V. Kretova, Ildar R. Alembekov, and Nickolai A. Tchurikov
RNAi has potential as an antiviral gene therapy strategy. Cassette constructs simultaneously expressing several siRNAs could prove to be the most efficient technique in developing gene therapy approaches for highly mutable viruses such as HIV-1.
Phosphorothioate-based DNA recombination: an enzyme-free method for the combinatorial assembly of multiple DNA fragments
Jan Marienhagen, Alexander Dennig, and Ulrich Schwaneberg
Rational guided generation of protein chimeras has developed into an attractive approach in protein engineering for tailoring catalytic and biophysical properties of enzymes.
Chao Wang, Jasmine Lee, Yinyue Deng, Fei Tao, and Lian-Hui Zhang
Current methods for identifying transcription start sites (TSSs) of specific genes in bacteria usually require adaptors or radioactive labeling. These approaches can be technically demanding and environmentally unfriendly.