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BioTechniques Virtual Symposium and Exhibit Hall

Methods are the foundation of any modern lab. They enable research, making it possible to experimentally test far reaching hypotheses quickly. With this in mind, the 2013 BioTechniques Virtual Symposium celebrated the creativity and imagination behind some of today’s most innovative methods, techniques, protocols and instrument advances. From enhanced PCR protocols to sophisticated imaging techniques enabling nanometer resolution, the sessions, posters and special keynote talks at this year’s event focused on the evolution of the key methodologies driving modern research.

Now Available on Demand:

Session 1 - Advancing Molecular Biology

Keynote Lecture on Cloning/Mutagenesis - Feng Zhang, Ph.D., Assistant Professor of Neuroscience, Massachusetts Institute of Technology; Core Member of the Broad Institute of Harvard University and MIT

The ability to create gene knock-outs or knock-ins has enhanced understanding of the role that genes play in a cell. Directed mutagenesis approaches have greatly expanded in recent years through development of platform technologies that take advantage of homologous recombination and nucleases. In addition, methods to clone large pieces of DNA have revolutionized fields such as synthetic biology (e.g. Gibson cloning). Dr. Zhang’s talk will focus on the development of nuclease-based genome engineering tools with an emphasis on the recently reported CRISPR-Cas system. View Today

The BioTechniques 30th Anniversary Lecture on Gene and Drug Delivery

During 2013, BioTechniques celebrates 30 years of publishing methods and techniques for life science researchers. To honor this special milestone, we have created the BioTechniques 30th Anniversary Lecture presented by Dr. Robert Langer, Sc.D. of the Massachusetts Institute of Technology. Over his career, Dr. Langer has been at the forefront of developing new methods for small molecule, drug and gene delivery. This special lecture will focus on various delivery methods and techniques, delivery vehicles and the impact these efforts have had for both basic science as well as in the development of clinical drug delivery systems. View Today

Session 2 - PCR in 2013

Keynote Lecture on qPCR - Jo Vandesompele Ph.D., Professor of Functional Genomics and Applied Bioinformatics, Ghent University

Quantitative PCR has become a staple in the modern molecular biology lab – a tool often used to measure quantities of expressed transcripts or numbers of specific RNAs in a sample. While an extremely useful tool if done properly, qPCR can present challenges when it comes to data interpretation and analysis. Dr. Vandesompele will examine the latest advances in qPCR methodologies and analysis tools. View Today

Lecture on Multiplex PCR - Larry Wangh, Ph.D., Professor of Biology, Brandeis University

The ability to multiplex PCR reactions can significantly extend the impact of PCR assays into areas such as gene expression analysis and even clinical diagnostics. However, multiplexing is limited by reagents, conditions and primer compatibility – factors that add to the difficulty of designing good multiplex PCR assays. Dr. Wangh’s talk will focus on the development of multiplex PCR applications and the use of LATE-PCR (a technique he developed). View Today

Lecture on Digital PCR - Kenneth Kinzler, Ph.D., Professor of Oncology, Johns Hopkins University. Director, Ludwig Center at Johns Hopkins

The ability to precisely determine template numbers using digital PCR approaches has provided novel insights into the molecular world. In this talk, Dr. Kinzler will describe his lab's development and use of digital PCR to understand the genetics of cancer. View Today

Workshop on Plastic Labware Leaching

Recent scientific reports have shown that chemicals used in the manufacturing of disposable plastic labware, such as slip agents, plasticizers, and biocides, can leach out of the plastic into your sample and affect bioassay results. This seminar gives a summary of how it can affect your experiments. View Today

Session 3 - Understanding Modern Biochemistry

Keynote Lecture on Protein Isolation -Scott Banta, Ph.D., Associate Professor of Chemical Engineering, Columbia University

To study a protein, a researcher much first identify and isolate that protein. Dr. Banta will detail recent work from his lab involving novel protein purification approaches and new protein engineering strategies, highlighting their possible applications for biological research. View Today

Lecture on Protein Analysis - Keith Weninger, Ph.D., Associate Professor of Biophysics, North Carolina State University

So, you think you have your pure protein, now what? This second talk will examine the latest techniques for protein quantification and functional analysis. Dr. Weninger will describe recent work from his lab using FRET-based approaches to examine protein conformational changes in living cells. View Today

Lecture on Protein Structure - Fang Tian, Ph.D., Assistant Professor of Biochemistry and Molecular Biology, Penn State University

Once you have that protein and know it’s concentration, functional capabilities, and relative purity, then what? Structure analysis enables researchers to suggest further functional properties of unknown proteins and group seemingly different proteins into families. Dr. Tian will discuss the recent efforts of his lab to elucidate the structure, function, and dynamics of membrane proteins using NMR spectroscopy. View Today

Session 4 - Modern Cellular Imaging: Seeing is Believing

Keynote Lecture on Light Microscopy Advances - Stephen Paddock, Ph.D., Howard Hughes Research Fellow at the University of Wisconsin

Light microscopy continues to evolve. Recent months have seen the advent of specialized objectives for deeper multi-photon imaging as well as reagents that clarify samples for deeper imaging. And the speed of confocal imaging continues to increase. Dr. Paddock will address recent advances in confocal imaging applications as well as other developments in light microscopy. View Today

Lecture on Fluorescent Probes and Proteins - Mark Rizzo, Ph.D., Assistant Professor, University of Maryland

Imaging is nothing without something to image. Low numerical aperture (NA) objectives and high-resolution imaging are crucial to many modern cell biology experiments. But these techniques need fluorescent probes or dyes to work. Dr. Rizzo will discuss his lab’s use of a structure-based approach to design brighter and more stable fluorescent proteins as well as FRET-based sensors. View Today

Lecture on High-content Imaging - Steven Altschuler, Ph.D., Associate Professor and Endowed Scholar in Biomedical Research, University of Texas Southwestern Medical Center

We will end the 2013 VS with a special talk focusing on the development of high-content imaging. HCI is now a mainstay for many screening applications in the pharma world, and is increasingly used by basic scientists as well. Dr. Altschuler will describe his recent development of a new computational tool that enables the rapid profiling of microscopy images from high-content screening applications. View Today

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