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Cell Culture: Why Does It Remain Difficult? | BenchTalk, Episode 10

Maintaining cells outside of their natural environment is no easy task, but advances in microfluidics might change the way cell culture is performed in the future.

In this episode, researchers James Piret from the University of British Columbia and Aaron Wheeler from the University of Toronto discuss the present and future of cell culture methods.

And in our science roundtable, science writers Sarah C.P. Williams and Ashley Yeager discuss the #overlyhonestmethods Tweets and research misconduct apologies and rehab.

Guests
	James Piret is a professor at University of British Columbia.
	Aaron Wheeler is the director of the Wheeler Microfluidics Laboratory at the University of Toronto.
	Sarah C.P. Williams is a freelance science writer based out of Kailua, Hawaii. You can follow her latest work at http://www.facebook.com/sarahcpwilliams.
	Ashley Yeager works in the Office of News and Communications at Duke University and is also a freelance science writer. You can follow her at https://plus.google.com/104786459743651414888/about.
	Andrew S. Wiecek is news editor at BioTechniques and host of the monthly podcast BenchTalk. You can follow him at http://www.biotechniques.com/news.
Related Links
	James Piret At the University of British Columbia, James Piret is a professor in the Chemical and Biological Engineering Department and the Genome Science and Technology Department. In a paper published in PLoS One in 2012, Piret and colleagues developed a method to culture purified pancreatic cells, providing a new model for pancreatic cancer and diabetes research. During the Cell Culture Engneering XIII conference in April 2012, Piret received the 2012 Cell Culture Engineering Award. John Morris and Jason Steel At the University of Toronto, Aaron Wheeler is head of the Wheeler Microfluidics Laboratory where he develops digital microfluidic devices for cell culture applications. In 2012, Wheeler has received the Arthur F. Findeis Award, the Joseph Black Award, and the Heinrich Emanuel Merck Prize. To see how digital microfluidics works, check out the Wheeler Microfluidics Laboratory YouTube page. Roundtable In an article published by BioTechniques in July 2012, Sarah C.P. Williams wrote about a $37 million dollar DARPA project to develop organ-on-chip devices for drug toxicity tests. In article published by BioTechniques in May 2012, Ashley Yeager wrote about a study that found that the amino acid glycine is consumed in larger than normal quantities by fast-growing cancer cells. The recent trending hashtag #overlyhonestmethods on Twitter included several overly honest cell culture posts. The most popular stories on BioTechniques in January 2013 was about a neuroscientist who apologized for falsifying data, as reported by Jesse Jenkins. In a story covered by Nature News, science writer Daniel Cressey wrote about a program led by St. Louis University ethicist James DuBois called Restoring Professionalism and Integrity in Research.