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In Focus

Sanger sequencing

Sanger sequencing has been the powerhouse of DNA sequencing since it was invented by Frederick Sanger in 1977, a creation for which he won his second Nobel Prize in Chemistry in 1980. ...

DNA methylation oxford nanopore in focus

Human whole-genome sequencing

The rise of whole-genome sequencing over the last decade, and its subsequent increase in prevalence throughout basic and clinical research, has been well documented. Frequently, the ...

Neuroscience

One of the key research challenges in neuroscience involves study of the blood-brain barrier and discovery of its role in the pathology of diseases of the central nervous system (CNS). ...

Introducing NGS primer extension target enrichment

Next-generation sequencing (NGS) revolutionized the life sciences and has given rise to fields such as clinical sequencing, advanced our understanding of genome-typing and gene ...

Advanced proteomic analysis

The impressive speed of development in the realm of genomic techniques has dramatically expanded our understanding of disease risk and highlighted key indications for the biology behind ...

The evolution of PCR

qPCR is a well-established and relatively straight-forward diagnostic method that, as the most accurate method of testing available, has been used extensively during the course of the ...

Western blotting

Western blotting forms the bedrock of many protein studies, with the technique cited in 9% of all protein related papers. The technique had its 40th birthday in 2019, an event that was ...

Developing molecular therapeutics

Molecular-based therapeutics are growing in prevalence due to the advantages that they present, for instance, the longer lasting, more targeted impact of their activity. In the ...

Advances in HPLC

High-performance liquid chromatography (HPLC) is a popular and well-established technique that is used to separate, identify and quantify each component in a sample. The technique has ...

Infectious disease sample collection

Infectious disease research is an essential component of human society that has been thrown into the spotlight by the COVID-19 pandemic. This research is enabled by a diverse array of ...

Realizing precision medicine with whole-genome sequencing

When techniques for whole-genome sequencing (WGS) first gained prominence, they revitalized the field of genomics, launching global efforts such as the 100,000 Genomes Project and ...

Environmental sequencing for COVID-19

During the COVID-19 pandemic, sequencing has been used extensively to study the genome of the virus, trace the origins of infections and examine the alterations in novel variants. One ...

Reproducibility in microbiomics

The field of microbiomics has expanded rapidly over the past decade, making its way into studies of human health, disease, the environment, agriculture, and many others. Worldwide ...

COVID-19 diagnostics

As we begin 2021, vaccines providing immunity to COVID-19 are beginning to be rolled out and it feels as though hope is on the horizon. However, vaccine programs will take time to ...

Reproducibility

Reproducibility is a topic that has been much discussed, debated and repeated over the last decade as the scale of the ‘reproducibility crisis’ has become apparent. In recent years, ...

Neuroscience

This In Focus will bring to light the science behind imaging the brain, both in patient neuroimaging and brain tissue samples. Focusing on both ends of the spectrum, we will discuss the ...

Big data

With our increasing capabilities in research techniques and tools comes an inevitable increase in the amount of data and the complexity of datasets. This in turn brings about the need ...

RNA Sequencing for Cancer Research

The capabilities of RNA sequencing, such as the insight into global transcriptional events, coding mRNA transcripts and RNA species profiles, have allowed RNA Sequencing to grow into a ...

DNA Sequencing

DNA sequencing has developed in leaps and bounds since the completion of the Human Genome Project in 2003. This In Focus feature will include: hot tips for next-generation sequencing ...