Coupling Flow Cytometry With an Expanded Palette of Fluorescent Proteins to Enhance Gene Expression Analysis
Free Web Event
Advances in flow cytometry and a full spectrum of fluorescent proteins now available allow biomedical researchers to more quickly, easily and affordably leverage this technology in gene expression analysis. Fluorescent proteins have come a long way since the original application of Green Fluorescent Protein (GFP) for the detection of gene expression. Fluorescent proteins now span the entire spectrum from short violet to long red, and can be used to study a wide variety of cellular phenomena. Personal flow cytometry simplifies the detection of fluorescently tagged genes incorporated into cells.
In the first segment of this webinar, we’ll review how fluorescent proteins and flow cytometry are utilized in gene expression analysis. The second portion will discuss strategies for the analysis of gene expression in angiosperms including a unique method for preparation of plant nuclei suspensions, the combination of natural or transgenic expression of fluorescence to identify specific cell types, and selective purification using cell sorting for subsequent gene expression analysis. The third portion will discuss how bacterial strains expressing fluorescent protein fusion constructs can be used to characterize the environment – gene regulation relationship and to quantify populations according to the resulting gene expression.
In this webinar you will learn:
- How flow cytometry techniques and a full spectrum of fluorescent proteins enable more efficient gene expression analysis.
- How the application of a gene expression workflow involving upstream flow cytometry for targeting, subsequent sorting for purification and downstream molecular analysis can speed gene expression analysis for different types of cells, including plant, bacterial and mammalian.
- How the BD Accuri C6 flow cytometer can be easily integrated into the laboratory for fast analysis, even for those with little flow cytometry experience
Patrick C.H. Lo, Ph.D.
Associate Editor, BioTechniques
Director of the Flow Cytometry Core Laboratory in the NCI Experimental Transplantation and Immunology Branch
Professor of Plant Sciences within the BIO5 Institute at the University of Arizona
Assistant Professor at the University of Houston
Director of Marketing, Personal Flow Cytometry Platforms / BD - Accuri Cytometers
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