Protocols
BioTechniques Protocols section is a collection of detailed, optimized procedures and protocols for use in the lab. You have access to detailed product information in a downloadable PDF format-which can be easily added to a notebook for ready access.
Protocol categories
2010 BioTechniques Protocol Guide
BioTechniques' Protocol Guide 2010
is a collection of detailed, optimized procedures/protocols for use in the lab. With BioTechniques' Protocol Guide, you have access to detailed product information in a downloadable PDF format-which can be easily added to a notebook for ready access. Each protocol is accompanied by a link to the company web site and an e-mail link to contact the company directly.
Featured Protocols
Immunostaining
Ricardo B. deMedeiros, Ph.D. and Joy Aho, Ph.D.
.
Vendor-submitted protocol
.
Sponsored by
R&D Systems, Inc.
.
Published in
November
2009
Flow cytometry is a powerful technique for assessing the expression of multiple proteins simultaneously in individual cells within a heterogeneous population...
Latest Protocols
Small RNA
Vendor-submitted protocol.
Sponsored by
Applied Biosystems, part of Life Technologies
Published in
BioTechniques 2010 Protocol Guide
Lipofectamine® RNAiMAX Transfection Reagent is a proprietary, animal
origin–free formulation for the transfection of siRNA into eukaryotic cells
with low cytotoxicity. Silencer® Select siRNAs are high-performing
siRNA molecules that incorporate the latest innovations in siRNA design,
chemical modifications, and off-target effect prediction algorithms.
Peer-Reviewed Protocols
Wai Hung Tsang and King L. Chow
Peer-reviewed, author-submitted protocol.
Published in
November
2009
pp55-
56
Procedure 1. A pair of fine forceps with a region of ~1 mm in width is used to hold the thin end of an autoclaved gel-loading tip in a perpendicular position (Figure 1).
Peer-Reviewed Protocols
Kim M. Linton1,5*, Yvonne Hey2*, Sian Dibben2, Crispin J. Miller3, Anthony J. Freemont4, John A. Radford1,5, and Stuart D. Pepper2
Peer-reviewed, author-submitted protocol.
Published in
November
2009
Protocol overview
• This protocol covers tissue sectioning and macrodissection steps in preparation for RNA extraction from FFPE tissues. Although originally developed for RNA extraction using the Optimum FFPE RNA Isolation Kit (Ambion, UK), the protocol is relevant for FFPE tissue preparation for RNA extraction using any extraction method
• Macrodissection is carried out at 2× magnification using a dissecting microscope with external fiber-optic illumination
• Adopt strict RNase-free handling conditions throughout, including the use and frequent changing of powder-free latex gloves.
Peer-Reviewed Protocols
Kim M. Linton1,5*, Yvonne Hey2*, Sian Dibben2, Crispin J. Miller3, Anthony J. Freemont4, John A. Radford1,5, and Stuart D. Pepper2
Peer-reviewed, author-submitted protocol.
Published in
November
2009
Protocol overview
• The extraction method (steps 2–21) is taken from the method supplied with TRIzol reagent (Invitrogen, Paisley, UK).
• Recover tumor tissue at the time of surgery, trim into 1-cm3 fragments, and immerse immediately in TRIzol reagent prior to freezing at -80°C.
Peer-Reviewed Protocols
Michael A. Green1, Shannon Bass2, and Brett T. Spear1,3
Peer-reviewed, author-submitted protocol.
Published in
November
2009
Instructions for use
For the production of zygotes and pseudopregnant females to serve as recipients, standard transgenic methodologies are used. Matings are set up with male and female mice as in standard transgenic procedures.
Peer-Reviewed Protocols
Yong-Chul Jung1, Jia Xu2, Jun Chen1, Yeong C. Kim1, David J. Winchester3, and San Ming Wang1
Peer-reviewed, author-submitted protocol.
Published in
November
2009
pp46-
47
Depending on the desired resolution and sequencing scale, different restriction enzymes can be used for genomic DNA digestion. PstI is used here as an example.
From Soil/Environmental Samples
Vendor-submitted protocol.
Sponsored by
Zymo Research Corporation
Published in
November
2009
Metagenomics (otherwise known as environmental genomics, ecogenomics, or community genomics) is the study of genetic material recovered directly from environmental samples (e.g., soil). This relatively new field of genetic research focuses on the study of organisms that are not easily cultured in the laboratory and studies of organisms in their natural environment.
Methylation Detection
Vendor-submitted protocol.
Sponsored by
Zymo Research Corporation
Published in
November
2009
High Resolution Melt (HRM) analysis can be used to differentiate DNAs based on variations in primary sequence for applications such as genotyping, SNP analysis, and DNA methylation analysis. Zymo Research has developed the EZ qPCR/HRM PreMix, featuring a robust hot-start polymerase and an intense dsDNA binding dye, for streamlined and precise qPCR and HRM applications.
Immunostaining
Ricardo B. deMedeiros, Ph.D. and Joy Aho, Ph.D.
Vendor-submitted protocol.
Sponsored by
R&D Systems, Inc.
Published in
November
2009
Flow cytometry is a powerful technique for assessing the expression of multiple proteins simultaneously in individual cells within a heterogeneous population. This technique is most commonly used with non-adherent cells, but it can also be an effective tool for analyzing protein expression in adherent cells.
Protein Extraction Isolation and Purification
Vendor-submitted protocol.
Sponsored by
Promega Corporation
The HaloTag® Protein Purification System is based on a unique protein tag, HaloTag®, which allows covalent, efficient, and specific capture of proteins expressed in Escherichia coli. HaloTag® was engineered from a bacterial dehalogenase to enhance expression and solubility of recombinant proteins and to provide efficient protein purification coupled with tag removal.
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