Have advances in LC-MS/MS guided the era of single-cell proteomics? Find out in your latest issue of BioTechniques!

In this issue of BioTechniques, we present an extraction method for sampling cucumber phloem sap, a detection method of SARS-CoV-2 variants of concern and a protocol using flow cytometry and Sanger sequencing to measure the edits from CRISPR/Cas9 on a B-lymphoblast cell line.

Technology News

Advances in LC-MS/MS usher in the era of single-cell proteomics

Expert Opinion

Legionella water testing and the EU Drinking Water Directive: could potentially harmful Legionella bacteria slip through the gaps?

Reports

Extraction of cucumber phloem sap based on the capillary-air pressure principle

Intracellular flow cytometry complements RT-qPCR detection of circulating SARS-CoV-2 variants of concern

Cloning vectors for gene delivery, integration and expression in Campylobacter jejuni

Isothermal amplification using sequence-specific fluorescence detection of SARS coronavirus 2 and variants in nasal swabs

Benchmark

Optimized RNA isolation of FFPE uterine scar tissues for RNA expression analyses delineated by laser microdissection

Evaluation protocol for CRISPR/Cas9-mediated CD19 knockout GM24385 cells by flow cytometry and Sanger sequencing

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