Application Note: Alternative transcript isoform detection with single-cell and spatial resolution
Transcript isoforms play a vital role in heath and disease, but studying them has been challenging due to limitations in traditional methods. By combining long nanopore sequencing reads with single-cell assays, researchers can identify full-length transcripts with single-cell resolution.
This Application Note introduces a new library preparation method compatible with 10x Genomics and Oxford Nanopore Technologies’ PromethION device to enable more efficient single-cell calling and clustering by gene expression and cell-type annotation.
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In this Application Note explore:
- Oxford Nanopore Technologies’ sequencing compatibility
- How to resolve cell barcoding and UMI assignments
- How full-length reads allow clustering by isoform expression and detection of alternative transcripts with single-cell and spatial resolution
And much more!
This content was provided by Oxford Nanopore
Discover more about single-cell sequencing with Oxford Nanopore:
- Case study: Explore new dimensions of biology with single-cell nanopore sequencing
- Blog: Applying single-cell long nanopore sequencing reads to identify the genetic basis underlying resistance in leukaemia treatment
- Webinars: Single-cell transcriptomics with Oxford Nanopore
- Workflow: Obtaining full-length isoforms from single cells with long nanopore sequencing reads
