Are technical replicates a necessity in RT-qPCR? – Find out in your latest issue of BioTechniques

In this issue of BioTechniques, we present the development of a BRET-based senor for the detection and quantification of interactions involving regulatory subunit B56α, an assay for monitoring mitophagy flux in live muscle cells and a method for determining the relationship between cellular RNA and protein expression levels in bronchoalveolar lavage samples.

Reports

Dual compartment utility of BRET-based biosensors for PPP2R5A/B56α, a cancer-associated B regulatory subunit of PP2A

Mito-Kaede photoactivation and chase experiment for mitophagy: mitophagy flux response toward various stimulations

Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage

Assessing the necessity of technical replicates in reverse transcription quantitative PCR

An enzyme-free alcohol-based organoid harvesting solution

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