Are technical replicates a necessity in RT-qPCR? – Find out in your latest issue of BioTechniques

In this issue of BioTechniques, we present the development of a BRET-based senor for the detection and quantification of interactions involving regulatory subunit B56α, an assay for monitoring mitophagy flux in live muscle cells and a method for determining the relationship between cellular RNA and protein expression levels in bronchoalveolar lavage samples.
Reports
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage
Assessing the necessity of technical replicates in reverse transcription quantitative PCR
An enzyme-free alcohol-based organoid harvesting solution
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