Measuring Cell Division in PMBCs by Flow Cytometry
Cell proliferation rates provide insights into relative cell health and are a widely used diagnostic indicator for numerous cancer types. In immunology, measuring cell proliferation is particularly useful for assessing immune responses such as T cell activation. In the past, cell proliferation assays involved radioactive labelling of DNA, but today fluorescent dyes such as ViaFluor® SE and CFSE are a safer and easier alternative for tracking cell replication in vivo and in vitro.
Cell proliferation of peripheral blood mononuclear cells (PMBCs) can be a useful read-out of immune cell activation. In their latest Tech Tip, Biotium outlines a typical procedure for using fluorescent ViaFluor® SE Dyes with PMBCs, which can easily be adapted for use with other cell types.
In this Tech Tip, you will learn:
- A typical procedure for using fluorescent ViaFluor® SE dyes for measuring lymphocyte proliferation in peripheral blood mononuclear cells.
- Key tips for getting the best results from your assays.
- Key considerations for proliferation dye and viability stain selection.
And much more!
This content was provided by Biotium
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