Technique Tales | Cellular annotation: the keystone of spatial analysis

For spatial biology to deliver on its potential, foundational aspects of the spatial analysis workflow need to be executed with precision. Here, Kalins Banerjee and Evan Keller explain the importance of cellular annotation, highlighting the challenges in this process and the solutions available to help surmount them, before detailing the techniques and tricks they use in their own approach to cellular annotation. Spatial transcriptomics is an emergent and transformative field that combines the high-resolution mapping of gene expression with the architectural context of tissues, opening new vistas in the study of cellular heterogeneity and tissue organization. Unlike conventional single-cell RNA...