Improving heterologous protein production in yeast with massively parallel CRISPR genome editing
Development of microbial strains for heterologous protein production necessitates a whole-genome engineering approach. In order to achieve the optimal performance, engineering efforts should include not only editing of the heterologous protein expression cassette, but genomic targets, such as transcription and translation machinery, transport and signaling, protein degradation and other pathways that contribute to the productivity and robustness of the host strain.
To engineer production strains rapidly and cost-effectively, genome-wide editing libraries must be generated in multiplex and provide trackability to connect phenotype to genotype. An innovative genome engineering technology – the Onyx™ Digital Genome Engineering Platform – performs genome-wide and trackable CRISPR editing at scale in an automated benchtop device. This approach significantly reduces the time and resources spent on strain development and optimization, as demonstrated in this Application Note.
In this Application Note, you will learn about:
- Using a combination of informed and exploratory approaches to strain engineering for heterologous protein production
- Design, construction, and screening of 14,859 diverse genome-wide edits to improve production of a fungal cellobiohydrolase I (CBH1) enzyme in S. cerevisiae
- Identification of 74 unique functional targets for improving protein production, including transcription, translation, secretion, degradation, stress response, and more
This content was provided by Inscripta.