Scientific Brief: Ultrafiltration devices and plates offer higher-throughput options for cloning and PCR workflows

This Scientific Brief describes the use of ultrafiltration devices in various steps of the cloning workflow that can provide a more streamlined process and compares them against traditional methods of subcloning PCR-generated fragments.

The use of ultrafiltration devices, particularly 96-well filter plates, enabled significant savings in time, labor, and starting materials, with numbers of transformed recombinants that roughly equaled those obtained with traditional techniques.