BioTechniques journal October November December 2020

October 2020 – Vol. 69 No. 4

  • November 2020 ‐ Vol. 69 No. 5
  • December 2020 ‐ Vol. 69 No. 6

Best of BioTechniques eBook – October to December 2020

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Highlights from this issue include:

From the Editor: Looking back on 2020 and forward to 2021

Tech news: 

  • Developing drugs for the ‘undruggable’
  • CRISPR vs COVID-19: how can gene editing help beat a virus?
  • How has the COVID-19 pandemic impacted PCR?

Reviews: Antibody validation for protein expression on tissue slides: a protocol for immunohistochemistry

Reports in brief:

  • High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
  • A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates
  • Methods for simultaneous and quantitative isolation of mitochondrial DNA, nuclear DNA and RNA from mammalian cells
  • A clinically validated human capillary blood transcriptome test for global systems biology studies
  • Critical role of three-dimensional tumorsphere size on experimental outcome
  • Reduced serum methods for contact-based coculture of human dermal fibroblasts and epidermal keratinocytes
  • RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors

Benchmarks in brief:

  • DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen
  • Quality control and statistical evaluation of combinatorial DNA libraries using nanopore sequencing
  • Comparison of target enrichment strategies for ancient pathogen DNA

Plus new products for your lab, information on our cover competition, and many more method articles, interviews and expert opinion pieces. 

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