Comparison of Agilent Femto Pulse system sizing with long-read sequencing read length
While long-read sequencing methods provide a number of advantages over short-read NGS approaches, they can be challenging due to the preferential sequencing of short fragments. In this study, the Agilent Femto Pulse system was used to address these challenges by successfully analyzing gDNA quality and size. Sheared samples, with and without size selection, were analyzed with the Agilent Femto Pulse system, and then sequenced on the Oxford Nanopore Technologies MinION. This crucial information can help determine appropriate size-selection thresholds and aid in quality assessment for samples prior to downstream analysis.
More information about long-read sequencing using the Agilent Femto Pulse system is available:
- Nanopore Sequencing Webinar | Agilent
- Fast Separation. Ultra Sensitivity. One Instrument. Agilent Femto Pulse System
In this Application Note you will learn more about:
- Sample shearing
- Quality control of size-selected gDNA to confirm selection
- Comparison of average smear size and mean sequencing read length
And much more!
This content was provided by Agilent.
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