Is the proof in the protocol when it comes to reproducibility? Find out in your latest issue of BioTechniques! 

Written by Ebony Torrington (Managing Editor)
Cell and tissue biology PCR and sequencing Proteomics
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In this issue of BioTechniques, we describe a PTC-on system to tune gene expression in a dose-dependent mode at translation level, a method to isolate extracellular vesicles from tissues based on frozen section pretreatment to obtain tissue fragments and compare three extraction kits for the isolation of DNA and RNA to evaluate the specificity and isolation efficiency. 

Technology News

Reproducibility in the lab: the proof is in the protocol 

Reports

Effect of mismatch between types of viral nucleic acid and intended targets of extraction kits on polymerase chain reaction-based testing 

Premature termination codon: a tunable protein translation approach 

A cryostat-based frozen section method to increase the yield of extracellular vesicles extracted from different tissues 

Benchmark

Gel purification of gDNA for next-generation sequencing applications 

A building block-based beam-break (B5) locomotor activity monitoring system and its use in circadian biology research 

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