Sanger sequencing for SARS-CoV-2
Despite advances in next-generation sequencing technology, Sanger sequencing remains the ‘gold standard’ for sequence validation. Sanger sequencing is particularly useful for detecting and confirming mutations within the SARS-CoV-2 genome and conducting variant surveillance. This Application Note provides a protocol for sequencing a region of the SARS-CoV-2 genome from nasal swab samples, using a new terminator sequencing dye with analysis by capillary electrophoresis.
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In this Application Note you will learn:
- The materials required for Sanger sequencing of SARS-CoV-2
- The protocol to follow for Sanger sequencing of SARS-CoV-2
And much more!
This content was provided by Promega.