Analytical validation of an error-corrected ultra-sensitive ctDNA next-generation sequencing assay

High-throughput multigene assays interrogating circulating tumor DNA (ctDNA) have the potential to significantly improve the field of precision oncology. However, existing methods are expensive and lack the sensitivity required to identify ultra-rare somatic variants. In this protocol, the authors (Fettke et al., The University of Melbourne, Australia) present a next-generation sequencing assay that uses molecular barcoding to achieve a high sensitivity and specificity for ctDNA interrogation, while mitigating the high costs associated with a high proportion of unusable sequencing reads.

View the full protocol on next-generation sequencing assays here